Effect of SJAMP on apoptosis of human hepatocellular carcinoma cell line HepG2 and the expression of Bcl-2, nm23-H1 in vitro

VernacularTitle:刺参黏多糖诱导人肝癌细胞凋亡的实验研究
Author: Xibao SUN ; Baolei WANG ; Jiahong LIU ; Bingyuan ZHANG ; Yun LU
Publication Type:Journal Article
Keywords: Stichopus japonicus acid mucopolysaccharide; HepG2; Bcl-2; nm23-H1
From: International Journal of Surgery 2010;37(5):303-306
CountryChina
Language:Chinese
Abstract: Objective Through studying the apoptosis induced by stichopus japonicus acid mucopoly saccharide in the hepatocellular carcinoma cell line HepG2 in vitro, analysing the expression of Bcl-2 and nm-23in HepG2, to provide the theory foundation and its feasibility on whether it can be used for the chemotherapy of hepetocellular carcinoma. Methods The cells of HepG2 were cultured in vitro and treated with SJAMP at different doses(0.25,0. 5,1.0,2.0,4.0 g/L). MTT was used to observe the inhibitory effects of SJAMP on cell growth, Western blotting was used to detect apoptosis, and the apoptosis related change of expression of protein Bcl-2 and nm23-H1. Results (1) MTT identified that SJAMP produced an obvious time-and-dose-dependent inhibitory effect on the HlepG2 cells. (2) Western blot showed that SJAMP could induce the apoptosis of HepG2 cells through changing the expression of the protein of Bcl-2 and nn23-H1 (P<0.05). Conclusion (1)SJAMP produced obvious inhibitory effects on HepG2 cells and induce HepG2 apoptosis. (2)SJAMP can enduce the anti-tumor function in the method of changing the expression of protein Bcl-2 and nm23-H1.