The effects of Salvia-miltiorrhiza concentration in gelatin-bletilla/Salvia-miltiorrhiza material on angiogenesis in rats
10.3760/cma.j.issn.1671-0282.2010.05.005
- VernacularTitle:丹参浓度对明胶-白芨胶/丹参材料促血管再生作用的影响
- Author:
Geliang HU
;
Wei JIN
;
Rui PENG
;
Lin CAI
- Publication Type:Journal Article
- Keywords:
Gelatin-bletilla;
Salvia-miltiorrhiza;
VEGF;
Histocompatibility;
Angiogenesis
- From:
Chinese Journal of Emergency Medicine
2010;19(5):466-469
- CountryChina
- Language:Chinese
-
Abstract:
Objiective To evaluate the histocompatibility of gelatin-bletilla/Salvia-miltiorrhiza i material and to discuss the appropriate concentration of salvia-miltiorrhiza in the material. Method Salvia-miltiorrhiza together with gelatin and bletilla was used to make a kind of porous membranes. A total of 175 rats were divided randomly (random number) into 5 groups ( n = 35 in each). The porous membranes containing salvia-miltiorrhiza material in different concentrations of 1, 2, 4, and 5 mL/100 g were implanted subcutaneously into the rats of the four experimental groups, respectively. The gelatin-bletilla material without salvia-miltiorrhiza was implanted into the rats of control group. In 1, 3, 7, 14, 21, 28 and 56 days after implant, the materials were taken out of the bodies of the rats, separately. The material implanted for 1, 3, 7 and 14 days were observed with immunohistoche-mistry. Results In the area of implant, no noticeable inflammation or other was found. The implanted materials were degraded and entirely absorbed in vivo in 8 weeks. The salvia-miltiorrhiza in concentration of 2 mL/100 g in vivo promoted the angiogenesis most significantly. Conclusions The gelatin-bletilla/salvia-miltiorrhiza material has excellent histocompatibility and the appropriate concentration of salvia-miltiorrhiza in material is 2 mL/100 g.
