The relationship between the change of ET-1 ,NOS and neuronal function score of cerebral vasospasm after experimental rat subarachnoid hemorrhage
10.3760/cma.j.issn.1674-6554.2010.05.004
- VernacularTitle:实验性大鼠蛛网膜下隙出血后脑血管痉挛神经功能评分与血浆内皮素、一氧化氮合酶水平的关系
- Author:
Zhiping CAI
;
Zhaohui LI
;
Jinsong ZHU
;
Huixian CUI
;
Nan LI
;
Lei XUE
- Publication Type:Journal Article
- Keywords:
Subarachnoid hemorrhage;
Vasospasm;
Endothelin-1;
Nitric oxide synthase
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2010;19(5):394-396
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the relationship between the change of endothelin-1 (ET-1), nitric oxide synthase(NOS) content and cerebral vasospasm after experiment Sprague-Dawley rat subarachnoid hemorrhage (SAH). Methods SD rat got the tail artery blood and followed prone positioning in a stereotaxic apparatus, injected autologous arterial blood or saline into cisterna magna,2 d later injected in the same way. For basilar artery (BA),internal carotid artery(ICA) ,and middle cerebral artery(MCA) diameter measurements,gelatin-india ink vascular casted,the blood ET-1 and NOS content were detected by immunoradiology or colorimetry measurement. Results Microangiography in SAH showed extensive macroscopic filling deficit,the diameter of MCA,ICA and BA ((169.33 ±8.67)mm, (227.33 ± 14.25) mm, (226.33 ±5.99) mm respectively) were shorter than those in control group((259.5 ±7.48)mm,(228.17 ±8.09)mm,(254. 67 ±8.48)mm respectively) (all P<0.05). Neuronal function score of 7d(9.45 ±1.77) was higher than control group(0.60 ±0.49) (P<0.05). Content of ET-1 and NOS in blood after SAH 7 d were(231.25 ± 19.45)g/L,(198.50 ±9.52) × 103U/L) ,(72.99 ±5.83)g/L, (230.76 ±17.06) × 103U/L),for sham group,there was significant differences (P<0.05). Conclusion Experiment rat double-hemorrhage method from tail artery can makes experimental subarachnoid hemorrhage model,neurological functional score is increased, neuronal function score is associated with diameter of MCA and ET-1 content.
