Apoptosis vs necrosis in hyperlipidemic acute pancreatitis in rats

VernacularTitle:高脂血症促使大鼠急性胰腺炎胰腺腺泡由凋亡趋向坏死
Author: Yonghui TANG ; Yi MIAO ; Zipeng LU ; Shilong FU
Publication Type:Journal Article
Keywords: Pancreastitis; Hyperlipidemia; D-fructose; Caspase-3,caspase-8
From: Chinese Journal of Hepatobiliary Surgery 2010;16(5):381-384
CountryChina
Language:Chinese
Abstract: Objective To establish the model of hyperlipidemie acute pancreatitis(AP)to explore the mechanism of hyperlipidemia in aggravating AP.Methods Sprague-Dawley rats were divided into four groups at radmon:1)control group(C);2)hyperlipidemic group(TG);3)acute pancreatitis group(AP);4)hyperlipidemic acute pancreatitis group(HAP).After 8 weeks,blood,pancreas and peripancreas tissue were collected from each rats for determination of various parameters.Results The levels of hyperilidemic index were elevated in HAP group compared with those of other groups.The ADP/ATP ratio was obviously increased in HAP group.Caspase-3 and caspase-8 of HAP group presented in zymogen forms,while in AP group they were in activity forms.After the pancreas issue were stained bv HE and further analyzed through TUNEL assay,we found that there were more pancreatic acinar cells undergoing necrosis in HAP group.Conclusion Hyperilidemia may promote the course of apoptosis to necrosis of pancreatic acinar cells,which may be the mechanism of deterioration in hyperlipidemic acute pancreatitis.