Effects of siRNA targeting transforming growth factorβ1 on biological characteristics of rat hepatic satellite cells
10.3760/cma.j.issn.1008-1372.2010.05.007
- VernacularTitle:转化生长因子β1小干扰RNA对肝星状细胞生物学特性的影响
- Author:
Rongquan FU
;
Deming JANG
;
Jiguang DING
;
Jinguo WU
;
Liang HONG
;
Qingfeng SUN
;
Qiang LI
- Publication Type:Journal Article
- Keywords:
Transforming growth factor betal/GE/ME;
RNA,small interfering;
Hepatocytes/ME
- From:
Journal of Chinese Physician
2010;12(5):596-599
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of TGFβ1 siRNA on hepatic satellite cells (HSCs) activation, proliferation and extracellular matrix production. Methods The TGFβ1, siRNA plasmid was transfected into HSCs with Lipofectamine 2000. The supernatant and HSCs were collected after incubation for 72h. The expression of TGFβ1, and a-SMA protein in HSCs was detected by. Western blotting. The expression of type Ⅰ and Ⅲ collagen mRNA was detected by RT-PCR. The cell proliferation was assayed by MTT method. Contents of typeⅣ collagen and hyaluronic acid in supernatant were determined by radioimmuno-assay.Results Compared with scrambled control group, the TGFβ1, and a-SMA protein expression,the activity of HSCs proliferation,the expression of typeⅠ and Ⅲ collagen mRNA,and the contents of type Ⅳ collagen and hyaluronic acid in supernatant were reduced in TGFβ1, siRNA group by (79±5)%,(55±4)%, (25±4)% ,(63±6)% ,(57±4)% ,(53±8)% ,(46±8)% ( P<0.01),respectively.Conclusion TGFβ1, siRNA could significantly reduce the expression of TGFβ1,inhibited HSC activation,proliferation and extracellular matrix production.
