Patent-blue-liposomes for lymphography and the detection of their biological property

VernacularTitle:淋巴造影剂专利蓝脂质体的制备及其生物学表征
Author: Jianzhong MA ; Chaofu HOU ; Youcheng ZHANG ; Zhihua LIU
Publication Type:Journal Article
Keywords: Patent blue; Liposomos; Lymph-mapping developer
From: International Journal of Surgery 2010;37(6):380-382
CountryChina
Language:Chinese
Abstract: Objective To prepare patent-blue-lipesomes(PB-LPS),a lymph-mapping developer.Methods PB was encapsulated in the liposomes using the passive loading method.The female Wistar rats were injected subcutaneously with the PB-LPS,PB,blank liposomes or isotonic rmtrium chloride at the heel solos,to study the stained situation of the lymph nodes and chemical analysis oftheir ALT,AST,BUN,Cr.Results The lymph nodes were obviously blue staining after injected PB-LPS or PB,the blue smimng ratios were 40%,20%(P<0.05),and the effect of PB-LPS on tracing of lymphy nodes was better than PB.No significant difference was found in ALT,AST,BUN and Cr.Conclusion The PB-LPS can be successfully prefabricated with such advantage as avirulence and high entrapment efficiency by passive loading method.