Effects of 5-azacytidine and estradiol on the methylation of CpG motifs and expression of DNA methyltransferase 1 in patients with SLE
10.3760/cma.j.issn.0412-4030.2010.06.016
- VernacularTitle:5-氮杂胞苷与雌二醇对SLE患者CpG基序甲基化状态与DNMT1表达的影响
- Author:
Xiaohua ZHU
;
Jun LIANG
;
Yongsheng YANG
;
Jinhua XU
;
Leihong XIANG
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus,systemic;
Methylation;
CpG islands;
Azacitidine;
Estradiol
- From:
Chinese Journal of Dermatology
2010;43(6):415-417
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of 5-azacytidine and estradiol on the methylation of CpG motifs and expression of DNA methyltransferasel (DNMT1) mRNA in patients with systemic lupus erythematosus (SLE) and normal controls.Methods Peripheral blood lymphocytes isolated from 12 patients with SLE and 11 normal human controls were stimulated with phytohaemagglutinin for one day followed by additional 3-day culture with or without the presence of 5-azacytidine of 1 μmol/L or estradiol of 30μg/L respectively.Then.the methylation of CpG motifs was detected by flow cytometry using anti-5-methylcytosine antibody,and DNMT1 mRNA expression by real time reverse transcription-PCR Results After treatment with 5-azacytidine,a decrease wag observed in the methylation of CpG motifs, but not in the expression of DNMT1 mRNA in peripheral lymphocytes from patients with SLE (1=18.60,P<0.01;t=1.56.P>0.05) and in those from the normal controls (t=5.63,P<0.01;t=2.17,P>0.05) compared with untreated lymphocytes.Nevertheless,there were no significant changes in the methylation of CpG motifs or expression of DNMT1 mRNA in lymphocytes from patients with SLE (t=1.53,0.93,respectively,both P>0.05) and normal controls (t=1.93,0.11,respectively,both P>0.05) after the treatment with estradiol.Conclusions The methylation of CpG motifs is suppressed efficiently by 5-azacytidine,and the suppression is unlikely to be associated with the decrease of DNMT1 mRNA.Estradiol has no significant impact on the methylation of CpG motifs and expression of DNMT1 mRNA in lymphocytes.
