Effects of 5-aza-dc on the expression of insulin-like growth factor binding protein 7 (IGFBP7) in and proliferation of melanoma cells

VernacularTitle:5-氮-2'-脱氧胞苷对黑素瘤细胞IGFBP7表达及细胞增殖的影响
Author: Yanning XUE ; Hao CHEN ; Wuqing ZHOU ; Yali SONG ; Xuesi ZENG ; Jianfang SUN
Publication Type:Journal Article
Keywords: Melanoma; 5-Aza-2'-deoxycytidine; Insulin-like growth factor binding protein 7
From: Chinese Journal of Dermatology 2010;43(6):418-421
CountryChina
Language:Chinese
Abstract: Objective To study the effects of 5-aza-dc on the expression of IGFBP7 in and proliferation of melanoma cell lines A375 and M14.Methods Reverse transcription-PCR and immunocytochemistry were performed to detect the mRNA and protein expression of IGFBP7 in A375 cells and M14 cells after treatment with 5-aza-dc of 10μmol/L for 48 hours,and MTT assay to measure the proliferation of both cell lines treated with 4 different concentrations (2.5,5,10,20μmol/L) of 5-aza-dc for various durations.Results The treatment with 5-aza-dc restored IGFBP7 expression at both mRNA and protein levels.The four concentrations of 5-aza-dc inhibited the proliferation of A375 and M14 cells in a dose-dependent (F=561.12,271.43,respectively,both P<0.01) and time-dependent (F=141.35,549.33,respectively,both P<0.01) manner.Conclusions DNA methylation may be involved in the modulation of aberrant IGFBP7 gene expression in melanoma,and 5-aza-dc could inhibit the proliferation of A375 and M14 cells.