Cetuximab enhances the radiosensitivity of radioresistant human esophageal squamous carcinoma cell line KYSE-150R
10.3760/cma.j.issn.0254-5098.2010.03.016
- VernacularTitle:西妥昔单抗对人食管癌放射抗拒性细胞株KYSE-150R放射敏感性的研究
- Author:
Haiying LI
;
Li ZHANG
;
Jianbo WU
;
Huafang SU
;
Shixiu WU
- Publication Type:Journal Article
- Keywords:
Esophageal carcinoma;
Radiosensitivity;
Cetuximab
- From:
Chinese Journal of Radiological Medicine and Protection
2010;30(3):291-294
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effect of cetuximab(C225)on the radioresistant human esophageal squamous carcinoma eell line KYSE-150R.Methods A radioresistant human esophageal squamous carcinoma cell line KYSE-150R was established by fractionated irradiation.Morphological changes from KYSE-150 to KYSE-150R were observed by phase-contrast microscopy.Karyotype analysis was performed by G-banding.The radiosensitivities were analyzed by colony formation assays.Results The population doubling time of KYSE-150 and KYSE-150R were(23.6±0.2)h and(25.9±0.6)h (t=6.6,P<0.01),respectively.The chromosome number of KYSE-150R was increased and chromosome aberrations were observed from(69.3±1.9)h to(73.7±1.2)h(t=-8.83,P<0.01).The SF2,D0,Dq and N values of KYSE-150R were all higher than those of KYSE-150.After 5μg/ml of C225 added,the SF2,D0,Dq and N values were significantly decreased as compared to the control.After C225 treatment,the G0/G1 and G2/M phase cells were increased,while S-phase cells decreased(t=-4.478-4.308,P<0.05).Conclusion Cetuximab can enhance the radiosensitivity of radioresistant human esophageal squamous carcinoma cell line KYSE-150R.
