The role of hIL-10-MSCs in apoptosis of rat discordant liver xenotransplantation
10.3760/cma.j.issn.0254-1785.2010.06.013
- VernacularTitle:输注转染人IL-10基因的骨髓间充质干细胞减轻大鼠异种移植肝脏的细胞凋亡
- Author:
Jian NIU
;
Bin LIU
;
Yewei ZHANG
;
Xiangnong LI
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
Interleukin-10;
Liver transplantation;
Apoptosis
- From:
Chinese Journal of Organ Transplantation
2010;31(6):369-372
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of hIL-10-MSCs on apoptosis of rats subject to discordant liver xenotransplantation. Methods The model of guinea pig to rat discordant liver xenotransplantation was set up. The orthotopic liver transplantation model was established by using modified two-cuff technique. Following groups were designed: control group, MSCs group and hIL-10MSCs group. Before and after operation, the recipients in control group were injected with normal sodium (2 ml) and decaesadril (0.5 ml) ;Those in MSCs group were injected with MSCs (4.0× 106/ml)and decaesadril (0.5 ml); Those in hIL-10-MSCs group were injected with hIL-10-MSCs (4.0 × 106/ ml)and decaesadril (0.5 ml). Twelve h after operation, the livers of recipient were observed by HE staining and electron microscope, and apoptosis of the livers was detected by using TUNEL. The expression levels of hIL-10, caspase-3, Fas and FasL were assayed by Western blot or immunohistochemistry. Results As compared with control group and MSCs group, the pathological changes was alleviated, the expression of IL-10 was significantly increased, the expression of FasL was significantly reduced in hIL-10-MSCs group. The positive area size of Fas and Caspase-3 in hIL-10MSCs group was 11.5 % and 25.1 %, respectively, significantly smaller than those in control group (35.3 % and 70.8 % respectively, P＜0.05. Apoptosis index in hIL-10-MSCs group was 32.5 %,which was significantly lower than in control group (74.1%) and MSCs group (50. 3 % ). ConclusionhIL-10-MSCs can notably decrease apoptosis of the transplanted liver probably by inhibiting theexpression of Fas/FasL.
