Application of double-lableling technique between in situ hybridization with cRNA probe and immunohistochemistry
10.3760/cma.j.issn.1008-6706.2010.11.003
- VernacularTitle:原位杂交与免疫组织化学双标记技术的应用价值
- Author:
Shuling ZHU
;
Qingxue YANG
- Publication Type:Journal Article
- Keywords:
In situ hybridization;
Immunohistochemistry;
RNA,complementary
- From:
Chinese Journal of Primary Medicine and Pharmacy
2010;17(11):1447-1448
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the double-labeling method of in 9itu hybridization(ISH) with cRNA probe and immunohistochemistry in the paraffin sections of the skin.Methods The double-standard coexistence signals by situ hybridization and immunohis- tochemistry in 15 cases with acne and folliculitis were observed.Results The blue-violet positive signals of ISH could be observed in the hair follicle epithelium,sebaceous glands and ducts of paraffin section of skin.Meanwhile,the brown dendritic positive signal also could be seen in the sebaceous glands.Conclusions The use of in situ hybridization with digoxin-labeled cRNA probe combined with immunohistochemistry could locate microbial component and epidermal cells on skin paraffin section exactly.
