The determination of a new single nucleotid polymorphism intron 5 (+4668) with high-resolution melting technology in gout patients
10.3760/cma.j.issn.1007-7480.2010.06.005
- VernacularTitle:运用高分辨率熔解曲线技术检测痛风患者SLC22A12基因intron5(+4668C/T)多态性
- Author:
Shuangchun LIU
;
Ming GUAN
;
Yuming CHEN
;
Jiong ZHANG
;
Xinju ZHANG
;
Xiaoye GU
;
Weizhe MA
;
Hejian ZOU
- Publication Type:Journal Article
- Keywords:
Hyperuricemia;
Polymorphism,single nucleotid;
Gout;
SLC22A12;
URAT1
- From:
Chinese Journal of Rheumatology
2010;14(6):377-380
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate a new single nucleotid polymorphism (SNP) intron5(+4668C/T) in SLC22A12 in primary gout patients and the association between clinical characteristics and genotypes. Methods One hundred and one primary gout patients and 186 healthy subjects were recruited into this study. Blood pressure, body mass index (BMI) was recorded. Serum uric acid, glucose, lipid and creatinine were detected. DNA was extracted from peripheral blood to amplify the fragment located in intron 5. The genotypes of SLC22A12 can be detected with high-resolution melting (HRM) assay, followed by sequencing analysis. Chi-square test was used for statistical analysis. Results ① A new SNP in intron 5 of SLC22A12 was identi-fied successfully by HRM, which was defined as intron 5 (+4668C/T). CC, CT and TT genotypes were unam-biguously distinguished with HRM technology, which was fully concordant with sequencing. ②The genotypes of CC, CT and TT in male and female groups were 28.1%, 33.7%, 38.2% and 20.0%, 47.1%, 32.9%, respectively.③ However, no significant differences of genotype distribution were found concerning BMI, blood pressure, creatinine, total cholesterol and triglyceride in both male group and female group. But the serum uric acid levels in the CC genotype were significantly higher than those with the CT+TT genotypes. ④ The genotype frequencies of CC and CT+TT in high uric acid group were remarkably different from those in low uric acid group (21.2%, 78.8%,; 35.0%, 65.0%; P<0.05). Conclusion A new SNP has been successfully discovered with HRM technology with simplicity, rapidity and accuracy. T allele of intron 5 (+4668C/T) may be a genetic protective factor for hyperuricemia among Chinese population.
