Relationship of PTEN expression level with positioning of AMPA receptor subunit GluR2 in hippocampal neuron after stretch injury

VernacularTitle:海马神经元损伤后PTEN表达水平与AMPA受体亚基表达定位变化的研究
Author: Minhui XU ; Yuan CHENG ; Chun ZHOU ; Guangjian SHEN ; Lunshan XU ; Liang YI
Publication Type:Journal Article
Keywords: Brain injuries; Genes; AMPA receptor; PTEN
From: Chinese Journal of Trauma 2010;26(6):551-555
CountryChina
Language:Chinese
Abstract: Objective To explore the relationship of phosphatase and tensin homology deleted on chromosome ten ( PTEN ) expression with the positioning of AMPA recepter subunit GluR2 in hippocampal neuron after stretch injury. Methods The primary rat hippocampal neuron from neonate rats were cultured and the model of stretch injury established. Western blot and TUNEL staining were used to detect PTEN expression and the positioning of AMPA receptor subunit GluR2 in rat hippocampal neuron at different time intervals after stretch injury. Results The PTEN expression was increased obviously after stretch injury, while the expressions of AMPA receptors GluR1, GluR2 and GluR3 showed no evident differences at every time point. However, AMPA receptor subunit GluR2 located at cytomembrane was down-regulated obviously after stretch injury. Conclusion The intracellular PTEN expression and the expression of AMPA receptor subunit GluR2 located at cytomembrane are on the contrary, indicating that PTEN phosphatase may participate in the transport and reintegration process of AMPA receptor subunit GluR2 located at cytomembrane.