Experimental therapeutic effect of combined cytokines on beagle dogs exposed to 4.5 Gy γ-rays
10.3760/cma.j.issn.0254-5098.2010.03.003
- VernacularTitle:细胞因子联合对4.5 Gyγ射线照射比格犬的实验治疗作用
- Author:
Shuang XING
;
Zhiqing HU
;
Ming LI
;
Haixiao HUANG
;
Hongling OU
;
Jianzhi ZHAO
;
Guolin XIONG
;
Ling XIE
;
Zhenhu ZHAO
;
Ning WANG
;
Jinxiang WANG
;
Ri ZHANG
;
Jingcheng MIAO
;
Nankang ZHU
;
Yuwen CONG
;
Qingliang LUO
;
Xueguang ZHANG
- Publication Type:Journal Article
- Keywords:
Cytokine;
Acute radiation sickness;
Recombinant human interleukin-11;
Recombinant human G-CSF;
Recombinant human interleukin-2;
Beagles
- From:
Chinese Journal of Radiological Medicine and Protection
2010;30(3):243-246
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of combined administration of recombinant human interleukin-11(rhIL-11),recombinant human G-CSF(rhG-CSF)and recombinant human interleukin-2 (rhIL-2)on acute radiation sickness(ARS)beagles.Methods Sixteen beagle were irradiated with 4.5 Gy60 Co γ-rays to establish ARS models,and were divided into irradiation control group,supportive care group and combined cytokines treatment group.After irradiation irradiation control group was given no treatment,the dogs in supportive care group received purely symptomatic treatment,while combined cytokines treatment group received rhIL-11 50μg/(kg·d)and rbG-CSF 10μg/(kg·d)subcutaneously(0-14 d)and rhIL-2 1×1 06 U/d(29-43 d)besides symptomatic treatment.Manifestation and characteristics of ARS beagles were observed,and the survival time were recorded.At last,post-mortem examination and histological examination were performed.Results All animals underwent nausea,diarrhea and fever.After irradiation,all animals in irradiation control group died in two weeks,and the mean survival time was 12.7 d,while only one died at 33 d in supportive care group.All dogs in combined cytokine group survived at 45 day after exposure,and their haematopoiesis and gastrointestinal tract were recovered.Conclusions Combination of rhIL-11 + rhG-CSF + rhIL-2 treatment could be significantly effective on ARS beagles irradiated by 4.5 Gy60 Co γ-rays,which could accelerate injured haemotopoiesis and intestinal tract recovery,increase the survival rate and improve the life quality of animals.
