Effect of ionizing radiation combined with inhibitors or inducer of autophagy and apoptosis on MCF7 cell

VernacularTitle:电离辐射联合自噬和凋亡抑制剂或诱导剂对MCF7细胞的影响
Author: Yali QI ; Jun WANG ; Yan LI ; Hongyan WANG ; Shouliang GONG
Publication Type:Journal Article
Keywords: Ionizing radiation; Autophagy; Apoptosis; Inductor; Inhibitor
From: Chinese Journal of Radiological Medicine and Protection 2010;30(3):263-266
CountryChina
Language:Chinese
Abstract: Objective To detect the inhibiting effects of ionizing radiation combined with inhibitors or inducer of autophagy and apoptosis on MCF7 cell line,and to provide the evidence for human breast cancer therapy radiation.Methods MCF7 cells were exposed to X-rays and randomly divided into 4 groups,including 0 Gy,4 Gy,4 Gy+rapamycin,4 Gy+3-MA,and 4 Gy+z-VAD-fmk groups,including 0 Gy,4 Gy,4 Gy+rapamycin,4 Gy+3-MA,and 4 Gy+z-VAD-fmk groups,respectively.The growth doubling time was calculated by MTT method.The specific protein expressions of LC3 autophagy and beclinl were detected by using Western blot and the difference of Drotein contents wasLC3 autophagy and beclinl were detected by using Western blot and the difference of Drotein contents was compared.The percentage of apoptosis of MCF7 cells was measured by flow cytometry (FCM).Resuits The growth doubling time of MCF7 cells in 4 Gy group wag longer than that in O Gy group(t=4.41,P