Analysis for expression and significance of interleukin-17, RORγt in CD4+ T cells from patients with ankylosing spondylitis
10.3760/cma.j.issn.1007-7480.2010.06.009
- VernacularTitle:强直性脊柱炎CD4+T细胞白细胞介素-17核孤儿受体的表达及意义
- Author:
Jinbing RUI
;
Jing LI
;
Lei CHEN
;
Haiyan YOU
;
Ling WU
;
Yingying QIU
;
Yanping LUO
;
Xiaoli SONG
;
Zhijun JIAO
- Publication Type:Journal Article
- Keywords:
Spondylitis,ankylosing;
Ienterleukin-17
- From:
Chinese Journal of Rheumatology
2010;14(6):391-393
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of IL-17, RORγt in CD4+T cells from patients with ankylosing spondylitis (AS). Methods The specimens of venous blood PBMC were collected from 28 patients with AS and 15 healthy subjects. Intracellular flow cytometry detection of IL-17 was established after isolation of human CD4+ T cells from PBMC. The expression level of IL-17, RORγt mRNA in CD4+ T cells was determined from 28 AS patients and 15 healthy controls by real-time fluorescence quantitative RT-PCR using Anti -CD3/Anti -CD28 as stimulators or not. Analysis of variance and Pearson correlation were selected. Results The isolation of human CD4+ T cells from PBMC was effective and its purity reached 90%. The percentage of intracellular IL-17 in CD4+ T cells from AS pati-ents in the AS active group was higher than that of the AS stable group and healthy control group (P<0.01). The expression level of IL-17, RORγt mRNA in CD4+ T cells was significantly higher in patients with AS than in controls. After stimulated with anti-CD3/ anti-CD28 stimulation, the percentage of IL-17, RORγt mRNA was increased significantly (P<0.01). The percentage of IL-17, RORγt mRNA in the 12 h group was higher than that of the 24 h group, while both of them were higher than those without stimulation (P<0.05). Conclusion There is an abnormal expression of IL-17, RORγt in human CD4+ T cells from AS patients. Our results indicate that the abnormal expression of IL-17 might play a role in the development and progression of AS.
