Expression of GLI1 and PTCH1 mRNA and yts correlation with clinical parameters in pancreatic cancer
10.3760/cma.j.issn.1674-1935.2010.03.016
- VernacularTitle:胰腺癌中GLI1、PTCH1 mRNA的表达及其与临床指标相关性分析
- Author:
Jiefang GUO
;
Jun GAO
;
Zhaoshen LI
;
Yanfang GONG
;
Jing JIN
;
Xiaohua MAN
;
Hongyu WU
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
GLI1;
PTCH1;
Neoplasm invasiveness;
Neoplasm metastasis
- From:
Chinese Journal of Pancreatology
2010;10(3):196-198
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of GLI1 and PTCH1 mRNA in pancreatic cancer and study its clinical significance. Methods Real-time fluorescence quantitative PCR (RFQ-PCR) was used to detect the expression of GLI1 and PTCH1 mRNA in 35 samples of pancreatic cancer tissues and 27 samples of adjacent normal pancreatic tissues, and the correlation of GLI1 and PTCH1 mRNA expression with clinical parameters was investigated. Results The relative expression of GLI1 mRNA in pancreatic cancer tissues was 1.12 ～ 3. 65 ( median 1.19), the relative expression of TCH1 mRNA was 1.82 ～ 4.36 ( median 2.36 ). The relative expression of GLI1 mRNA in adjacent normal pancreatic tissues was 0.23 ～ 2.76 ( median 0.87 ), the relative expression of PTCH1 mRNA was 1.11 ～ 2. 17 (median 0.58). Both the expression of GLI1 and PTCH1 mRNA in pancreatic cancer tissues were significantly higher than those in normal pancreatic tissues (P<0.05), and a positive correlation was found between GLIl and PTCH1 mRNA expression levels (P <0.05 ). The expression of GLI1 mRNA was significantly correlated with the differentiation degree and lymph node metastasis of pancreatic cancer (P < 0. 05). Conclusions GLI1 and PTCH1 may be involved in pancreatic carcinogenesis, and GLI1 may be related to invasion and lymph node metastasis of pancreatic cancer.
