Expression of microtubule-associated protein 1 light chain 3 following recurrent neonatal seizures in hippocampus
10.3760/cma.j.issn.1671-0282.2010.06.009
- VernacularTitle:微管相关蛋白1轻链3在新生期大鼠惊厥后海马的表达
- Author:
Hong NI
;
Yong GONG
;
Luyang TAO
- Publication Type:Journal Article
- Keywords:
Seizure;
neonatal;
LC-3;
Western blot;
Autophagy;
3- MA;
Hippocampus;
Rat
- From:
Chinese Journal of Emergency Medicine
2010;19(6):583-586
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the repetitive expressions of autophagy marker protein-rnicrotubule-associ-ated protein 1 light chain 3 (LC3) in hippocampus in newborn rats with recurrent seizure and the influence of 3-methyladeine (3-MA) on LC2 expressions. Method Seventy-two 6-day-old SD rats were randomly (random nam-ber) divided into the recurrent neonatal seizure group (RS group, n = 24), the 3-MA-treated seizure group (3-MA group, n = 24) and control group (n = 24). Rats in RS group were subjected to 55 attacks of seizure induced by flurothyl in 9 successive days from the 6th postnatal day (P6). In 3-MA group, 2 μL of 3-MA was injected every day till seizure induced. Western blot analysis was used to determine LC3 protein level in hippocampus at different intervals of 1.5 h,3 h,6 h and 24 h after the last convulsion. The LC3 protein level was analyzed with Dunnett test after ANOVA. Results LC3 protein levels in RS group at the different intervals were significantly higher than those in the control group and in 3-MA group (F =4.70,5.28,8.51 and 5.89, respectively, P <0.05), and there were no significant differences in LC3 protein level between 3-MA group and control group at those intervals (P > 0.05). Conclusions The autophagy/lysosomal pathway is immediately activated after recurrent seizure evidenced by the elevated expressions of LC3 in hippocampus. The 3-MA is involved in the regulation of autophagy/ lysosomal pathway by down-regulating the expressions of LC3.
