Reversal effect of sodium norcantharidate on human lung adenocarcinoma cell line A549/DDP in vitro
10.3760/cma.j.issn.0254-9026.2010.06.013
- VernacularTitle:去甲斑蝥酸钠对耐顺铂肺腺癌A549/DDP细胞的逆转作用及机制
- Author:
Suxiu CHEN
;
Yiyan JIANG
;
Zhenghua FEI
- Publication Type:Journal Article
- Keywords:
Antibiotic resistance,neoplasm;
Reverse Transcription
- From:
Chinese Journal of Geriatrics
2010;29(6):486-488
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the reversal effect and the mechanism of sodium norcantharidate(SNCTD)on human lung adenocarcinoma cell line A549/DDP. Methods CKK assay was used to screen out non-toxic concentration (less than 10 percent of cell inhibition ratio) of SNCTD, and to measure the IC50 of cisplatin and IC50 of innoxious concentration SNCTD plus cisplatin in drug-resistant cell line. The accumulation effect of Rh123 was assayed by flow cytometry after treatment with non-toxic concentration of SNCTD. PT-PCR was used to detect the expression of mdr1, MRP1 gene for the drug-resistant cell line treated with non-toxic concentration of SNCTD for 48h. Results (1)The non-toxic concentration of SNCTD was 5μg/ml. SNCTD could decrease drug resistance to cisplatin. The reversal fold was 1.97. (2)The fluorescence intensity of Rh123 in the cells treated with 5μg/ml SNCTD was obviously increased (F=36.99, P＜0.05). (3)The expressions of mdr1, MRP1 gene decreased significantly in a concentration-dependent manner.Conclusions SNCTD could reverse the resistance to cisplatin in A549/DDP cell line. It possibly downregulates the expression of mdr1, MRP1 gene, and inhibits the function of efflux pump of membrance protein.
