Effects of Pseudomonas aeruginosa vaccine on proliferation and invasiveness of hepatocellular carcinoma cell line MHCC97L
10.3760/cma.j.issn.1007-8118.2010.06.018
- VernacularTitle:绿脓杆菌制剂对人肝癌MHCC97L细胞增殖和侵袭能力的影响
- Author:
Jianwei ZHOU
;
Tao LI
;
Zhenggang REN
;
Binbin LIU
;
Xiuyan HUANG
;
Minjie JU
;
Zhaoyou TANG
- Publication Type:Journal Article
- Keywords:
Carcinoma hepatocellular;
Pseudomonas aeruginosa vaccine;
Proliferation;
Invasiveness;
VEGF;
MMP2
- From:
Chinese Journal of Hepatobiliary Surgery
2010;16(6):455-459
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Pseudomonas aeruginosa vaccine (PA) on proliferation and invasiveness of the hepatocellular carcinoma cell line MHCC97L with metastatic potential. Methods Proliferation, growth curve, plate efficiency, flow cytometry, transwell invasion assay, cell motility assay, scarification test, vascular endothelial growth factor (VEGF) and matrix metalloproteinase-2 (MMP2) protein activity were evaluated after cells were treated with PA at various concentrations. Results PA can inhibit the proliferation and plate efficiency of MHCC97L cell markedly in a dose-dependent manner. The IC50 of cells treated with PA for 48 h and 72 h was 3.1 ×108/ml and 1.9 × 108/ml, respectively. The doubling time increased and plate efficiency decreased gradually when cells treated with 0.5 × 108/ml, 1 × 108/ml and 2 × 108/ml PA (P＜0.01). PA could induce cell cycle arrest at the G1 phase in a dose-dependent manner by flow cytometric analysis. The average amount of invading cell per field in cell invasion assay and motility assay were 4. 8 ± 1.3 and 8. 8±2.2 when cells treated with 1× 108/ml PA, which was significantly lower than that of control group (8. 6±2. 1 and 15. 6±1.2 ) (P＜0.01) Scarification test showed that the metastatic ability of cells treated with 1 × 108/ml PA significantly lower than that in the control group. Comparison between cells treated with 1 × 108/ml PA and control group, no remarkable difference was found regarding expression of VEGF and MMP2 in supernatant of cell culture. Conclusion PA can inhibit proliferation and plate efficiency of HCC cell line MHCC97L, which is in part mediated by the cell cycle arrest at the G1 phase. PA could inhibit invasiveness of HCC cell line MHCC97L, which is unrelated to the VEGF and MMP2 protein activity.
