Upregulated expression of plasticity-related gene 1 protein in cerebral cortex in a rat model of recurrent neonatal seizures
10.3760/cma.j.issn.1674-6554.2010.06.009
- VernacularTitle:新生期大鼠反复惊厥上调大脑皮质可塑性相关基因-1蛋白水平
- Author:
Zhuojun XIAO
;
Hong NI
;
Zhedong WANG
- Publication Type:Journal Article
- Keywords:
Plasticity-related gene 1;
Recurrent seizures;
Cerebral cortex;
Developmental
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2010;19(6):504-506
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the dynamic expression of a new phospholipid phosphatase, plasticity related gene 1 ( PRG-1 ) in cerebral cortex following recurrent neonatal seizures.Methods A seizure was induced by inhalant flurothyl daily in neonatal Sprague-Dawley rats from postnatal day 6 (P6).Rats were assigned into the recurrent-seizure group (seizures induced in six consecutive days) and the control group.At 3 h,12 h,48 h,14d after the last convulsion,PRG-1 protein level in cerebral cortex was detected by western blot method.Results At 3 h, 12 h,48 h, 14d after the last convulsion in control group, the expression of PRG-1 in cerebral cortex respectively was 1.363 ± 0.742,1.278 ± 0.687 ,0.763 ± 0.374,1.004 ± 0.113, in experimental group , the expression of PRG-1 in cerebral cortex respectively was 1.818 ± 1.093,1.562 ± 0.782,1.024 ± 0.510,1.378 ± 0.279.At 3 h, 12 h,48 h, the expression of PRG-1 in cerebral cortex was not significantly different between the experimental and control group( t = 0.843,0.668,1.011 ,all P ＞ 0.05 ).However, at 14 d, the level of PRG-1 protein in cerebral cortex of experimental group was significantly higher than that of control ( t = 3.041, P ＜ 0.05 ).Conclusion The up-regulated expression of PRG-1 in cerebral cortex may be associated with the recurrent neonatal seizure-induced brain damage.
