The effects of dexamethasone on the over -expression of TGF-β_1 in the cerebral spinal fluid of rats following SAH
10.3760/cma.j.issn.1008-1372.2010.01.020
- VernacularTitle:地塞米松对蛛网膜下腔出血大鼠脑脊液TGF-β_1过度表达的影响
- Author:
Jicun WANG
;
Tong LI
;
Kai FENG
;
Jianxin WANG
- Publication Type:Journal Article
- Keywords:
Dexamethasone/PD;
Subarachnoid hemorrhage/DT/ME;
Transforming growth factor betal/ME;
Cerebrospinal fluid
- From:
Journal of Chinese Physician
2010;12(1):59-62
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the preventive and therapeutic effect of dexamethasone on communicating hydrocephalus following subarachnoid hemorrhage (SAH), and explore the mechanism of communicating hydrocephalus following SAH. Methods SAH rat model was constructed by injecting auto blood through foramen magnum into subarachnoid space. There were two groups ( one control group and one experimental group) in this study with 16 rats each group. Sufficient doses of auto blood were injected into subarachnoid space of the rats of the control group and sufficient dose of auto blood together with sufficient dose of dexamethasone into subarachnoid space of the rats of the experimental group. The changes of rats transforming growth factor bata-1 (TGF-β_1 )in the cerebrospinal fluid (CSF)and thickness of pia mater collagen fibrosis( CF) following SAH were observed after 1 day and 20 days. The levels of TGF-β_1, in the CSF were quantitatively detected by the way of ABC-ELISA. The brain tissues were dyed through Massions technique, and then the average thicknesses of meningina CF were measured by the software of micrograph collection and analysis computer system. Results In later stage after SAH, TGF-β_1, concentrations of the experimental group was lower than control group( P <0. 01). At the same time, the average thicknesses of meningina CF of experimental groups was thinner than control group( P <0. 01). Moreover, the relationship between the level of TGF-β_1 in the CSF and the thickness of the CF of the rat's cerebral pia mate had positive correlation. Conclusions In the later stage after SAH, dexamethasone can inhibit the over expression of TGF-β_1 in rat CSF following SAH and also can inhibit the accrementition of rat collagenous fibers. Therefore, this study provided experimental and theoretical evidence for preventing and treating communicating hydrocephalus following SAH.
