Differential radiobiological effects of carboxyfullerene C_3 on K562 and AHH-1 cells
10.3760/cma.j.issn.0254-5098.2010.01.001
- VernacularTitle:羧酸富勒烯C_3对K562和AHH-1细胞的辐射生物效应研究
- Author:
Hanchen LIU
;
Yu LI
;
Bailong LI
;
Jianguo CUI
;
Fu GAO
;
Ding SUN
;
Husheng SHAN
;
Fang ZHAO
;
Yuecheng HUANG
;
Junrui DONG
;
Hao LIU
;
Jianming CAI
- Publication Type:Journal Article
- Keywords:
Carboxyfullerene C_3;
K562 cell;
AHH-1 cell;
γ-ray;
Apoptosis
- From:
Chinese Journal of Radiological Medicine and Protection
2010;30(1):1-4
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the application prospective of carboxyfullerene C_3 as a radioprotectant or assistant for tumor radiotherapy.Methods Different concentrations of C_3 were incubated with K562 and AHH-1 cell,CCK-8 assay and trypan blue rejection test were performed to examine the influence of C_3 on the cell viability.Annexin V/PI staining and flow cytometry assay were applied to assess the cell cycle and apoptosis after 7-ray irradiation.Results C_3 showed little toxicity to AHH-1 cell with the survival rate over 95% ,but 600 mg/L of C_3 markedly inhibited the growth of K562 cell (82%) .Pretreatment of 100 mg/L C_3 significantly increased the survival rate of AHH-1 cell after 4 Gy irradiation compared with the single radiation group(71.3% vs 90.3%) ,but decreased the apoptosis rate (26.3% vs 12.6%) ,while the survival rate of K562 cell was decreased and the apoptosis rate was elevated with the increase of C_3 concentration.Moreover,the cell cycle analysis revealed the G_2 phase block in AHH-1 cell after radiation exposure was mitigated by C_3 pretreatment,but that in K562 cell was aggravated.Conclusions C_3 has good radioprotective effects on AHH-1 cells.For K562 cell,C_3 could inhibit the cell proliferation,promote the radiation induced apoptosis and aggravate the G_2 phase block.
