Detection of plasma PAF-AH and G994T single nucleotide polymorphism research in type 1 diabetes

VernacularTitle:PAF-AH基因G994T突变与1型糖尿病的关系研究
Author: Yuanyuan ZHANG ; Guanqi GAO ; Changgui LI
Publication Type:Journal Article
Keywords: Diabetes mellitus,type 1/GE; 1-alkyl-2 -acetylglycerophosphocholine esterase/GE; Mutation
From: Journal of Chinese Physician 2010;12(6):752-755
CountryChina
Language:Chinese
Abstract: Objective To investigate the plasma PAF-AH level in initialed type 1 diabetes patients and the association between the PAF-AH gene polymorphism and type 1 diabetes mellitus. Methods155 initialed type 1 diabetes patients and 138 controls were selected in this study. Plasma PAF-AH was determined by PAF-AH Assay kit, and the PAE-AH gene G994t genotypes were detected by PCR. ResultsThe activity of plasma PAF-AH in the patients with type 1 diabetes was significantly lower than that in the controls[ (20.64 ± 6.23)nmol/(min · ml) vs (28.56 ± 4. 11)nmol/(min · ml), P ＜0.05). Compared with control group, the frequencies of the GT genotype on G994T polymorphism between type 1 diabetes patients and controls were not significantly different(8.4％ vs 7. 2％, P ＞0. 05). ConclusionThe activity of plasma PAF-AH in the patients with type 1 diabetes was significantly lower than controls. The G994T polymorphism of PAF-AH gene was not related to type 1 diabetes mellitus.