Adaptive experiment of VNUKOVO-32 vcell culture rabies vaccine strain in cell lines
- Author:
Nguyen Thi Kieu Anh
- Publication Type:Journal Article
- Keywords:
Vnukovo-32;
Cell culture;
cell line;
Vaccine;
Experiment;
- MeSH:
Rabies Vaccines;
Cells
- From:Journal of Preventive Medicine
2005;15(5):128-132
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Vnukovo-32 (cell culture rabies virus vaccine strain) was propagated experimentally in primary hamster kidney cell (PHKC), primary Chick embryo fibroblast cell (CEF) and human diploid cell (WI-38). These 3 cell lines were infected with Vnukovo-32 by the same mount of infection (MOl) - lLD50/l000 cells. The result showed that the Vnukovo-32 was adapted on all experimental cell lines, met the criteria of master seed (the titer ranging 6.4-7.5 log LD50/ml), and reached the highest titer on the 7th - 9th day postinfection in three cell groups. Moreover, the highest titer of Vnukovo-32 was met in human diploid cell (WI-38), then in PHCK, and the lowest was in CEF. From these results, it is suitable to use WI-38 to propagate Vnukovo-32 for master and working seed. The procedure for propagation of Vnukovo-32 is set up.
