Co-culture of C3A cells and pancreatic endothelial cells and its effects on C3A cell functional changes
10.3760/cma.j.issn.1007-8118.2010.06.016
- VernacularTitle:胰腺内皮细胞共培养对C3A细胞功能变化的影响
- Author:
Zhiguo LI
;
Xueyi CUI
;
Yan WANG
;
Jiang DU
;
Yuzhan KANG
;
Yi GAO
- Publication Type:Journal Article
- Keywords:
Hepatocyte;
Pancreatic endothelial cell;
Co-culture;
Biological function
- From:
Chinese Journal of Hepatobiliary Surgery
2010;16(6):447-450
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the functional changes of C3A cells co-cultured with pancreatic endothelial cells. Methods C3A cells and pancreatic endothelial cells lwere cultivated directly and indirectly for 7 days by the density ratio of 10: 1. Meanwhile, the blank control was established.The growth and morphological characteristics of experimental groups were observed. The out leakage level of AST, ALT and the synthesis level of albumin and the diazepam metabolism level were determined by automatic biochemistry analyzer, radioimmunoassay and enzyme linked immunosorbent assay. Results Direct co-cultivation of C3A cells and pancreatic endothelial cells could reduce the out leakage of ALT and AST in the 5th and 7th d. The capacity of albumin synthesis could reach 12. 977μg/ml on the 7th d and the that of diazepam metabolism could also be improved. Indirect co-cultivation of C3A cells and pancreatic endothelial cells could significantly raise the albumin synthesis to 7. 380 μg/ml and 10. 773 μg/ml on the 5th and 7th d, respectively. Conclusion Co-cultivation of C3A cells and pancreatic endothelial cells can significantly improve the basic biological function of C3A cells.
