Effects of lipoxin A4 on inflammatory response to focal cerebral ischemia-reperfusion injury in rats
10.3760/cma.j.issn.0254-1416.2010.04.025
- VernacularTitle:脂氧素A4对大鼠局灶性脑缺血再灌注损伤时炎性反应的影响
- Author:
Xihong YE
;
Yan WU
;
Peipei GUO
;
You SHANG
;
Shanglong YAO
- Publication Type:Journal Article
- Keywords:
Lipoxins;
Brain ischemia;
Repedusion injury;
Inflammation
- From:
Chinese Journal of Anesthesiology
2010;30(4):465-468
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of lipoxin A4 (LXA4) on the inflammatory response to focal cerebral ischemia-reperfusion(I/R) inmy in rats.Methods Fifty-six healthy male SD rats weighing 200-250 g were randomly divided into 3 groups:group Ⅰ sham operation(group S,n=8);group Ⅱ cerebral I/R(n=24)and group Ⅲ lipoxin A4+I/R(group LXA4,n=24).Right mid-cerebral artery was occluded for 2 h by inserting cranially a nylon thread with rounded tip into internal carotid artery.LXA4 0.03 nmol/5 μl was injected into cerebral ventricle at 5 min after cerebral ischemia.Neurological deficit was scored at 24 h of reperfusion.Then four animals in each group were killed and their brains were removed for microscopic examination and expression of MPO at 24 h of reperfusion.Meantime,content of IL-1β,TNF-α,TGF-β1,and IL-10 in the brain tissue were measured at 1,6,12,24 and 48 h of reperfusion by ELISA.Glial cell activity was examined at 24 h of reperfusion by immuno-histochemistry.Results Intra-cerebroventricular administrated LXA4 0.03 nmol/5 μl provided mild neuroprotection against focal cerebral I/R injury,improved neurological deficits,and reduced morphological brain damages and PMN infiltration.LXA4 also decreased the content of TNF-α and IL-1β,and increased the content of IL-10 and TGF-β1.The numbers of activated astroglia and microglia were decreased in group LXA4 compared with group I/R.Conclusion LXA4 protects the brain against I/R injury by inhibiting inflammatory response.
