The expressions of TF mRNA and TFPI mRNA of liver in rats with Vibrio vulnificus sepsis and the intervention effects of antibacterial agents
10.3760/cma.j.issn.1671-0282.2010.07.015
- VernacularTitle:创伤弧菌脓毒症大鼠肝组织因子和组织因子途径抑制物的基因表达及抗生素的影响
- Author:
Huan LIANG
;
Zhongqiu LU
;
Qiaomeng QIU
;
Guangliang HONG
;
Mengfang LI
;
Tieli ZHOU
- Publication Type:Journal Article
- Keywords:
Vibrio vulnificus;
Sepsis;
Tissue factor (TF);
Tissue factor pathway inhibitor (TFPI);
Antibacterial agents
- From:
Chinese Journal of Emergency Medicine
2010;19(7):726-730
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions of TF mRNA and TFPI mRNA of liver in rats with Vibrio vulnificus sepsis and to assess the interventional effects of cefoperazone sodium along with levofloxacin lac-tate. Method One hundred and ten male SD rats were divided (random number) into normal control group (NC group, n = 10), Vibrio vulnificus sepsis group (VV group, five subgroups n = 10 in each), drug intervention model (AA group, five subgroups n = 10 in each). The Vibrio vulnificus sepsis models and drug intervention models of rat were made. The reverse transcription polymerase chain reaction (RT-PCR) assay was employed for the measurement of TF mRNA and TFPI mRNA. ANOVA and t-test performed with SPSS version 12.0 software. Results Compared with NC, the expressions of TF mRNA in liver increased markedly 2 h,6 h, 12 h and 16 h af-termodeling in VV groups (P<0.05), and reached peak 6 hours after modeling. The expressions of TF mRNA in liver of rats in AA groups were much higher than those in NC group 9 h and 12 h after modeling (P<0.05). The expressions of TFPI mRNA in liver of rats in VV groups and AA groups were not significantly different to those in NC group (P>0.05). Compared with VV groups, the expressions of TF mRNA in liver of rats in AA groups were greatly lowered 9 hours after administration of bactericide (P<0.05), and the expressions of TFPI mRNA in liver of rats in AA groups were significantly higher 12h and 16 h after intervention (P<0.05). Conclusions There is a obvious imbalance between coagulation and anticoagulation functions of circulation system during Vibrio vulnificus sepsis, and the imbalance can be corrected gradually after treatment with antibacterial agents.
