Proliferation of and production of interferon-γ by drug-specific peripheral T cells from pafients with severe drug eruption
10.3760/cma.j.issn.0412-4030.2010.07.014
- VernacularTitle:重症药疹患者外周血药物特异性T细胞扩增及干扰素γ产生的研究
- Author:
Yang GAO
;
Meng FU
;
Xiaowei SHI
;
Wenjun LIAO
;
Lin GAO
;
Tianwen GAO
;
Gang WANG
;
Chengxin LI
;
Yufeng LIU
- Publication Type:Journal Article
- Keywords:
Drug eruptions;
T-lymphocytes;
Interferon type Ⅱ
- From:
Chinese Journal of Dermatology
2010;43(7):478-481
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the proliferation of and production of interferon-γ by drug-specific peripheral T cells from patients with severe drug eruption.Methods Peripheral blood mononuclear cells (PBMCs) were isolated from 10 patients with severe drug eruption,10 patients with mild or moderate drug eruption and 10 normal human controls,stimulated with causative drugs to obtain drug-specific T cells.Then,both PBMCs and drug-specific T cells were stimulated with causative drugs or unrelated drugs followed by the detection of secretion levels of IFN-γ with ex vivo enzyme-linked immunodotting (ELISpot) assay and cultured ELlSpot assav respectively.Results After stimulation with causative drugs,a higher level of IFN-γ was secreted by PBMCs and drug-specific T cells from patients with severe drug eruption compared with those from normal human controls (both P<0.01).and by drug-specific T cells than by PBMCs (P<0.01).The culture with unrelated drugs could neither induce the generation of drug-specific T cells nor promote the secretion of IFN-γ by PBMCs from the patients.Drug-specific T cells still existed in the peripheral blood of 3 patients within 1 to 3 years after recovery of drug eruption.Conclusions There are drug-specific T cells in peripheral blood of patients with severe drug eruption,and they may persist for a certain period of time after recovery of drug eruption.Ex vivo ELISpot combined with cultured ELISpot may be applied to the identification of causative drugs in vivo.
