Effects of curcumin on mRNA expression of cytokines related to Tou-like receptor 4 signaling in THP-1 cells
10.3760/cma.j.issn.0412-4030.2010.07.019
- VernacularTitle:姜黄素对THP-1细胞Toll样受体4信号传导通路中相关因子mRNA水平的影响
- Author:
Lixin PENG
;
Xinyu LI
;
Shasha SONG
;
Yongfang WANG
;
Lanfang XU
- Publication Type:Journal Article
- Keywords:
Curcumin;
TNF receptor-associated factor 6;
Interleukin-1 receptor-associated kinases;
NF-kappa B;
Cell line
- From:
Chinese Journal of Dermatology
2010;43(7):493-496
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of curcumin on mRNA expression of cytokines related to Toll-like receptor (TLR) 4 signaling in THP-1 cells.Methods After pretreatment with different concentrations (50,25,12.5 mg/L) of curcumin or dexamethasone for 12 hours,THP-1 cells were stimulated by lipopolysaccharide (LPS.1 mg/L) for 4 hours followed by the collection of cells.Then total RNA was isolated from these cells and subjected to reverse transcription-polymerase chain reaction (RT-PCR) for the detection of mRNA expression of tumor necrosis factor receptor-associated factor 6 (TRAF6),interleukin-1 receptorassociated kinase (IRAK1) and nuclear factor (NF)-κB.THP-1 cells without pretreatment or stimulation served as negative control,and those only stimulated with LPS served as LPS group.Results After stimulation with LPS (1 mg/L) for 4 hours,the mRNA expressions of TRAF6,IRAK1 and NF-κB were significantly upregulated in THP-1 cells compared with negative control cells (f=38.69,39.13,23.99,all P<0.01).Curcumin of 50 mg/L and 25 mg/L significantly inhibited the mRNA expressions of TRAF6.IRAK 1 and NF-κB upregulated by LPS with an inhibition rate of more than 50% (all P<0.0 1).Conclusions Certain concentrations of curcumin can inhibit the mRNA expressions of TRAF6.IRAK1 and NF-κB.which demonstrates the regulatory effect of curcumin on the mRNA expressions of TLR4 signaling pathway-associated cytokines.
