Estimation of expanded criteria donor usability for islet isolation
10.3760/cma.j.issn.0254-1785.2010.07.005
- VernacularTitle:扩大标准的供者胰腺在胰岛移植中应用的动物实验
- Author:
Ying CHENG
;
Wenqing SONG
;
Ping MAO
;
Menglei ZHAO
;
Rui SHI
;
Yongfeng LIU
- Publication Type:Journal Article
- Keywords:
Islets of langerhans transplantation;
Pancreas;
Donor selection
- From:
Chinese Journal of Organ Transplantation
2010;31(7):397-399
- CountryChina
- Language:Chinese
-
Abstract:
Objective Islet transplantation has been an effective method for diabetes mellitus. The quality of donor pancreas is important for successful islet isolation. In this study, we evaluated expanded criteria donor usability based on the warm ischemic time, fatty pancreas and perfusion injury. Methods The marginal pancreases include those from cardiac death donor, fatty pancreas and edema pancreas from perfusion injury. Islets were isolated and purified using a modified University of Minnesota method. Islet yield and purity was determined by Dithizone (DTZ) staining and microscopic examination. Islet viability was assessed by AO/EB staining, and islet function was assessed by static glucose stimulation test. Results In the cardiac death donor group, the islet quality, viability, and in vitro function were similar when the warm ischemic time within 15 min. The quality and viability was decreased when the warm ischemic time beyond 30 min, but the function remained well. With 45 min warm ischemic time, insulin release index was decreased significantly. The islet quality, viability, and in vitro function from severe obesity group and severe edema group were decreased obviously. Conclusion Donor factors play a vital role in pancreas transplant outcomes. We concluded that pancreas severe obesity, severe edema and pancreas from cardiac donors (warm ischemic time ＞30 min) are unsuitable for islet isolation.
