Expression of Foxo3a in lumbar dorsal root ganglia after rat sciatic nerve injury

VernacularTitle:Foxo3a在大鼠坐骨神经损伤后背根神经节中的表达
Author: Youhua WANG ; Dawei XU ; Shuizhu XU ; Farui SUN ; Ran TAO
Publication Type:Journal Article
Keywords: Sciatic nerve; Ganglia,spinal; Regeneration; Foxo3a
From: Chinese Journal of Trauma 2010;26(7):631-634
CountryChina
Language:Chinese
Abstract: Objective To investigate the expression of Foxo3a in the lumbar dorsal root ganglia (DRG) after sciatic nerve injury in rats. Methods Forty-two adult rats were randomly divided into control group and experimental group. Rats in experimental group were subjected to sciatic nerve clamp. Expression and distribution of Foxo3a, growth associated protein (CAP) and axon regeneration in DRC were detected by Western blot and immunofluorescent staining. Results (1) After sciatic nerve injury, Foxo3a protein levels began to reduce at day 1, reached the lowest level at day 2 and then gradually rose to nomal level at four weeks after sciatic nerve injury. Proliferating cell nuclear antigen (PCNA) and GAP-43 were up-regulated from day 2, and the level of PCNA reached peak at day 7 after sciatic nerve injury in DRC. (2) Down-regulation of Foxo3a was observed predominantly in neurons and glial cells. PCNA mostly expressed in glial cells and hardly in neurons. Conclusion Down-regulation of Foxo3a in lumbar DRG may correlate with axonal regeneration and the proliferation of glial cells after sciatic nerve injury.