Effect of olfactory entheath cells transplantation on neurophysiological function and synaptic density in rats with traumatic brain injuries
10.3760/cma.j.issn.1001-8050.2010.07.022
- VernacularTitle:嗅鞘细胞移植对创伤性脑损伤大鼠神经功能和突触密度的影响
- Author:
Ke MAO
;
Hao LI
;
Jing CHENG
;
Hong ZHU
;
Chao YOU
;
Guoping LI
- Publication Type:Journal Article
- Keywords:
Brain injuries;
Cell transplantation;
Recovery of function;
Rats
- From:
Chinese Journal of Trauma
2010;26(7):644-647
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of olfactory entheath cells (OECs) transplantation on neural function and synaptic density of rats with traumatic brain injuries (TBI) and the possible mechanism. Methods Rats were divided into sham operation group, TBI group and OECs engrafted group. The brains of the rats were injured by Feeney percussion device through free falling. After cultured and identified by using specific marker (known as P75), OECs were transplanted into the area around the injured brain. Cortical somatosensory evoked potential (CSEP) and motor evoked potential ( MEP) were evaluated at day 14 after cell transplantation to determine the neurophysiologic function following TBI. Moreover, the synaptic densities around the injured brain were determined by using immunohistochemical method. One-way ANOVA test was used for statistical analysis. Results The transplanted OECs could survive and migrate around the injury site in the host brain 14 days after OECs transplantation. In addition, rats subjected to OECs implantation showed a marked neurophysiologic improvement and a significant increase of synaptic densities compared with the control group. Conclusion OECs transplantation can improve the neurophysiologic function and increase the synaptic density, which provides experimental basis for treatment of TBI with OECs.
