Proteomic analysis of alzheimer's disease Rats'serum
10.3760/cma.j.issn.1673-4181.2010.03.003
- VernacularTitle:老年性痴呆大鼠血清的蛋白质组学研究
- Author:
Huiyun YANG
;
Haixuan QIAO
;
Xin TIAN
- Publication Type:Journal Article
- Keywords:
Serum;
Alzheimer's disease;
Proteomics;
Complement component 4,gene 2
- From:
International Journal of Biomedical Engineering
2010;33(3):138-141
- CountryChina
- Language:Chinese
-
Abstract:
Objective Alzheimer's disease(AD) is a common and devastating disease and there is no readily available biomarker to aid diagnosis or to monitor disease progression. This study is to further understand the pathogenesis of Alzheimer's disease and seek new biomarkers for AD. Methods AD rats made by Aβ1-40 were assessed using the Morris water maze method. Sera of two groups were collected and albumin, IgG and sodium chloride were removed from the serum before concentration. Two-dimensional electrophoresis(2-DE) was performed on serum protein. Altered proteins were identified by matrix assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS). Results Compared with untreated samples, 10 altered proteins were found through 2-DE and all of them were down-regulated of which 2 were identified by MALDI-TOF-MS to be plasma retinol-binding protein precursor and complement component 4, gene 2 (C4b). Conclusion C4b may be the biomarker of AD and it may be helpful to understand the pathogenesis of AD.
