Effects of Tan ⅡA on expression of HIF-la and c-Met in gastric cancer SGC7901 cells under hypoxia

VernacularTitle:低氧下丹参酮ⅡA对人胃癌SGC7901细胞HIF-1α与c-Met表达的影响
Author: Yuguang FENG ; Xushan ZONG ; Guohui XING ; Yun ZHU ; Meiying WU
Publication Type:Journal Article
Keywords: Stomach neoplasms; Tanshinone Ⅱ A; Anoxia; Hypoxia-inducible factor 1
From: Journal of International Oncology 2010;37(5):394-396
CountryChina
Language:Chinese
Abstract: Objective To study the effects of Tan Ⅱ A on the expression of c-Met and HIF-lα in gastric cancer SGC7901 cells under hypoxia and the correlation between c-Met and HIF-lα expression. Methods The model of hypoxia was established by CoCl2. There were three groups; normal control group, hypoxia control group, and hypoxia combined with different concentrations of Tan ⅡA group. After Tan ⅡA was added to the media with 0.5, 2.0 and 10.0 mg/L respectively for 48 h under hypoxia, the expression of HIF-1α and c-Met protein was detected by immunocytochemical staining. Results Immunocytochemical staining revealed that the expression of HIF-1α and c-Met protein was inhibited by Tan ⅡA in a dose-dependent manner under hypoxia. High positive correlation was found between the expression of HIF-1α and c-Met (n = 4, r = 0. 996, P <0.01). Conclusion Tan ⅡA might inhibit the expression of c-Met protein via down-regulation of HIFlα protein expression under hypoxia, suggesting that Tan ⅡA might play an important role in fighting against hypoxia-stimulated invasion and metastasis of tumor.