Changes of function and ultrastructure of cerebral microvascular endothelial cells early after traumatic brain injury in rats

VernacularTitle:大鼠颅脑外伤后早期脑微血管内皮细胞功能和结构的改变
Author: Hui MA ; Zhiyuan QIAN ; Shengming HUANG ; Aidong WANG
Publication Type:Journal Article
Keywords: Brain injuries; Endothelial cells; Thrombomodulin; von Willebrand factor
From: Chinese Journal of Trauma 2010;26(7):635-639
CountryChina
Language:Chinese
Abstract: Objective To investigate the functional and ultrastructural changes of cerebral microvascular endothelial cells at early stage following traumatic brain injury (TBI) in rats. Methods The rat models with closed brain injury were established with the improved Marmarous method. The expressions of thrombomodulin (TM) and von Willebrand factor (vWF) were determined by immunohistochemical techniques (5 rats per group) and real-time quantitative RT-PCR (5 rats per group) respectively at 1, 4, 8, 12, 24 hours and at days 3 and 7 after injury. Results TM and vWF started expression at 4 hours, reached peak at 24 hours and recovered to normal at day 7 after TBI. The expression levels of TM and vWF at different time points in sham control group showed statistical difference compared with damage group (P < 0.05). Conclusion The activation of the cerebral microvascular endothelial cells at early stage after TBI is the main mechanism of early secondary brain injury.