Effect of brain derived neurotrophic factor precursor block on improvement of motor function in rats subjected to cord transaction

VernacularTitle:脑源性神经营养因子前体蛋白干扰促进脊髓全横断大鼠运动功能恢复
Author: Yinghua CHEN ; Hua JIN ; Qiqin DAN ; Jianguo QI
Publication Type:Journal Article
Keywords: Spinal cord injuries; Brain-derived neurotrophic factor; Recovery of function; Rats
From: Chinese Journal of Trauma 2010;26(7):653-656
CountryChina
Language:Chinese
Abstract: Objective To investigate the role of precursor of brain derived neurotrophic factor (proBDNF) in the improvement of motor function of rats subjected to cord transection. Methods Spinal cord transection was performed at T10 level. The rats were divided into sham operation group and artificial cerebrospinal fluid group. Then, the rats were subjected to proBDNF antibody treatment. The efficiency of proBDNF block was also determined by immunohistochemistry. BBB scale scores were used to e-valuate the effect of ProBDNF block on the motor function of the rats with spinal cord transection. Results Compared with control rats, proBDNF treatment showed a marked down-regulation of proBDNF protein in the spinal cord and could significantly improve the motor function. BBB scores in proBDNF block group was higher than those in artificial cerebrospinal fluid group. Conclusion The proBDNF block is available to the recovery of the motor function in hindlimbs in cord transected rats, suggesting that proBDNF could be as a member of candidate molecules for SCI therapy in the future.