Cognitive function improvement after administration of leptin into brain of rats with vascular dementia
10.3760/cma.j.issn.1674-6554.2010.07.015
- VernacularTitle:瘦素对血管性痴呆大鼠认知功能的改善作用
- Author:
Jiangying CHEN
;
Zhenwen YAN
;
Fusheng ZHANG
;
Suping ZHANG
- Publication Type:Journal Article
- Keywords:
Leptin;
Vascular dementia;
Cognitive function;
Apoptosis
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2010;19(7):621-623
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study improvement of cognitive function impairment of vascular dementia rats induced by a permanent bilateral ligation of common carotid arteries (2VO) after administration of leptin in brain. Methods Hippocampal neurons was isolated and cultured from SD rats. At oxygen-glucose deprivation condition, protection role of leptin on hippocampal neurons was observed and expression of leptin receptor was detected. Animal model of rats was established by 2VO. Pre-treatment VaD model of leptin was established by administration leptin into hippocampus region. The Morris water test was performed to detect difference in the cognitive function between VaD group and control group. Neuron apoptosis in hippocampus tissue were determined with TUNEL. Results Leptin receptor expression could be seen in hippocampal neurons. After oxygen-glucose deprivation cultured for 12 h , plenty of apoptotic cells were seen in hippocampal neurons, apoptosis rat was up to (72.96 ± 6.25) % , while apoptosis rate was (46.33 ±7.85)% and (23.58 ±5.08)% in 1 ( μg and 5μg leptin treatment group,respectively. Compared leptin treatment group with control group,difference had a statistical significance(P<0.01). Compared with untreated VaD group, latency time was shorter and average velocity was increased in leptin-treat-ment VaD group. Neuron apoptosis in hippocampus tissue of leptin-treated group were different significantly from those of untreated group (P < 0.01). Conclusion Leptin could protect hippocampal neurons from apoptosis in vitro. Cognitive function impairment could be improved by administration of leptin into brain in VaD rats.
