Relationship between the expression of HLA-DR,TLR4 in peripheral blood mononuclear cell and clinical outcome in severe sepsis patients
10.3760/cma.j.issn.1008-6315.2010.07.026
- VernacularTitle:重症脓毒症患者外周血单核细胞人类白细胞抗原和TLR4的表达与预后关系
- Author:
Hao GUO
- Publication Type:Journal Article
- Keywords:
HLA-DR;
TLR4;
Severe sepsis;
Peripheral blood mononuclear cell
- From:
Clinical Medicine of China
2010;26(7):743-745
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the change of expressions of human leukocyte antigen-DR (HLA-DR) ,pattern recognition receptor TLR4(toll like receptor 4) of peripheral blood mononuclear cell( PBMC) with severe sepsis and its relation to clinical outcome. Methods Thirty-five patients with severe sepsis and fifteen healthy volunteers were enrolled into this study. Thirty-five patients with severe sepsis were divided into the improved group (re =25) and the death group (n = 10).The protein expression level of HLA-DR,TLR4 on PBMC was analyzed by flowcytometry. Results The quantity of monocytes(0. 39 ± 0. 30) and the expression level of HLA-DR (10. 25 ± 5. 35), TLR4 (10. 93 ± 5. 66) on PBMC were significantly lower than those in healthy volunteers ( 0. 62 ± 0.41 ) , (59. 28 ±14. 76) and (39. 86 ±8. 55) pre-treat(P<0.01or P <0. 05).Along with the clinical condition improved, the quantity of monocytes and the protein expressions of HLA-DR, TLR4 on PBMC were gradually increased at each timepoint in the improved group. In this group the quantity of monocytes (1. 03 ± 0. 78) and TLR4 expression (36. 68 ± 16. 61 ) turned to the normal range in the 5th day, which were significantly higher than pre-treat (P < 0. 05 ). However,the expression failed to return to normal range at each time point in the death groups and there were no significant difference between each time point ( P > 0.05). Conclusions The quantity of monocytes and the expression level of HLA-DR,TLR4 on PBMC show significant relevance to patients' imnmnological function and the clinical outcome.
