Effects of CO2 pneumoperitoneum pressures on growth and proliferation of gastric cancer cells in nude mice transplanted tumor
10.3760/cma.j.issn.1007-631X.2010.07.022
- VernacularTitle:不同压力CO2气腹对胃癌细胞裸鼠移植瘤成瘤能力的影响
- Author:
Yingxue HAO
;
Peiwu YU
;
Chao ZHANG
;
Dongzhu ZENG
;
Yongliang ZHAO
;
Yan SHI
;
Yun RAO
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Pneumoperitoneum,artificial;
Cell proliferation;
Proliferating cell nuclear antigen;
Transplanted tumor
- From:
Chinese Journal of General Surgery
2010;25(7):572-574
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different CO2 pneumoperitoneum pressures on gastric cancer cells' growth and proliferation in nude mouse model of implanted tumor. Methods Human gastric cancer cell lines MNK-45 were exposed under 0、10、12 and 15 mm Hg CO2 pneumoperitoneum for 4 hrs respectively. 2 × 106 processed cells were inplanted into nude mice subcutaneously. Three weeks later, mice were sacrificed and the weight and bulk of the tumor measured. Then we observed the transplantation tumor by HE stain and Ki-67 stain. Results There was no significant difference in tumor's growing time, bulk and weight between 0, 10, 12 mm Hg CO2 pneumoperitoneum groups (7. 8 d, 7. 2 d, 7. 8 d; 1. 2 cm3, 1. 3 cm3, 1. 3 cm3; 1.5 g, 1. 9 g, 1. 6 g)and the control group (7. 3 d, 1. 2 cm3, 1.4 g) (P > 0. 05 ). The growing time of tumor in 15 mm Hg CO2 pneumoperitoneum (12. 5 d) was obviously longer than the control group ( P < 0.05 ) , the bulk and weight of tumor in 15 mm Hg CO2 pneumoperitoneum (0. 5 cm3, 0. 5 g) group significantly decreased compared with the control group (P <0.05). The positive rate of Ki-67 in 15 mm Hg CO2 pneumoperitoneum (27. 5% ) group was obviously lower than the control group (59.6%) (P<0.01). However, there were no significant differences between 0, 10, 12 mm Hg CO2 pneumoperitoneum groups (61.2%, 60.5%, 63.4%) and the control group (P > 0.05). Conclusion Clinically adopted CO2 pneumoperitoneum pressures have no significant effect on gastric cancer cells growth and proliferation.
