The sensitivity of cytopathic effect caused by influenza viruses in cultured Madin-Darby canine kidney cells

VernacularTitle:犬肾传代细胞分离流行性感冒病毒致细胞病变的敏感性
Author: Chenyan JIANG ; Shijiao NIE ; Xihong Lü ; Yingyang GAO ; Liwen JU
Publication Type:Journal Article
Keywords: Influenza A virus; Serial passage; Kidney; Cell line; Cytopathogenic effect,viral; Dogs
From: Chinese Journal of Infectious Diseases 2010;28(7):390-392
CountryChina
Language:Chinese
Abstract: Objective To understand the sensitivity of cytopathogenic effect (CPE) in MadinDarby canine kidney cells(MDCK) that cultured influenza A pharyngeal swab specimens of patients for one,two and three passages. Methods Influenza A pharyngeal swab specimens of patients were inoculated in MDCK for three blind passages. The presence of CPE of every passage was observed by inverted microscope. Results Of the 279 influenza A pharyngeal swab specimens of patients tested by colloidal gold, the presence of CPE in MDCK for one,two and three passages was 65.9%(184/279),91.4%(255/279) and 96.4%(269/279), respectively. Two hundred and seventy-one of 279specimens were identified as influenza A by multiplex reverse transcription-polymerase chain reaction (RT-PCR). Conclusion The positive separation rate can reach more than 95% by inoculating influenza A pharyngeal swab specimens of patients in MDCK for three blind passages.