The relationship of methylenetetrahydrofolate reductase G1793A gene polymorphism, hyperhomocysteinaemia and ulcerative colitis
10.3760/cma.j.issn.0578-1426.2010.08.011
- VernacularTitle:亚甲基四氢叶酸还原酶G1793A基因多态性及高同型半胱氨酸血症与溃疡性结肠炎的相关性
- Author:
Yi JIANG
;
Jie ZHAO
;
Changlong XU
;
Shuguang CAO
;
Limiao LIN
;
Yuan LEI
;
Sha HUANG
;
Changgao WANG
;
Bing XIA
- Publication Type:Journal Article
- Keywords:
Methylenetetrahydrofolate reductase;
Colitis,ulcerative;
Hyperhomocysteinemia;
Folic acid;
Vitamin B12;
Polymorphism,single nucleotide
- From:
Chinese Journal of Internal Medicine
2010;49(8):675-679
- CountryChina
- Language:Chinese
-
Abstract:
Objectives The present study aimed to investigate the associations between genetic polymorphism of methylenetetrahydrofolate reductase ( MTHFR) G1793A, plasma homocysteine (Hcy) levels, vitamin status and ulcerative colitis ( UC) in a cohort of patients in Hubei Han nationality. Methods Two hundred and ninty-nine UC patients and 764 age- and sex-matched healthy controls were recruited in this study. Polymorphism of MTHFR G1793A was examined using a PCR-RELP method.Plasma levels of Hcy, folate and vitamin B12 were determined by enzymatic cycling assay and corpuscle immune chemiluminescence assay, respectively.Results Both variant allele and genotype frequencies in MTHFR G1793A gene were significantly higher in the UC patients compared to the controls (22.24% vs 14.20% , P<0.001 ;42.81% vs 26.97%, P < 0.001, respectively).Plasma Hcy levels were increased in UC patients compared to the controls [(20.67 ±6.42)mmol/L vs (13.21 ±5.11)mmol/L, P <0.001] while folate and vitamin B12 concentrations were significantly decreased [(11.37±6.34) nmol/L vs (14.89±7.21) nmol/L, P < 0.001; (324.15±127.53 ) pmol/L vs (421.54±128.45 ) pmol/L, P < 0.001, respectively].Furthermore, hyperhomocysteinaemia (HHcy) and folate deficiency were also more prevalent in the UC patients (32.44% vs 25.78% , P = 0.029; 23.41% vs 17.01%, P =0.016, respectively).Conclusions Genetic polymorphism of MTHFR G1793A Wag strongly associated with UC.HHcy,folate deficiency and low vitamin B12 concentration were common phenomena in the UC patients of Hubei Han nationality.Our findings demonstrate that the genes relmed to Hey metabolism may play an important role in the pathogenesis of UC.
