Experimental study on bone formation with a novel borosilicate glass combined with platelet-rich plasma
10.3760/cma.j.issn.0253-2352.2010.08.012
- VernacularTitle:复合富血小板血浆的硼酸盐生物玻璃修复兔实验性骨缺损
- Author:
Yadong ZHANG
;
Gang WANG
;
Xin LIU
;
Wenchan HUANG
;
Changqing ZHANG
;
Zongpin XIE
;
Shengbao CHEN
- Publication Type:Journal Article
- Keywords:
Borates;
Biocompatiable materials;
Platelet-rich plasma;
Osteogenesis
- From:
Chinese Journal of Orthopaedics
2010;30(8):789-795
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the osteogenesis of a novel borosilicate glass materials combined with platelet-rich plasma in repairing segmental bone defects. Methods 36 New Zealand white rabbits which bilateral radius were resected into l.5cm bone defect, were divided into 4 groups averagely depending on implanted materials: group A (one side: D-Alk- 1B, another side: D-Alk- 1B +PRP), group B (one side:D-Alk- 1B +PRP, another side: β-TCP); group C (one side: β-TCP, another side: experimental bone defect),and group D(one side:D-Alk-1 B,another side :experimental bone defect). The specimens were examined after 4, 8,12 weeks; the osteogenesis was evaluated through gross observation, X-ray radiograph,histological examination,scanning electron microscope and Micro-CT. Results There were similar results about gross observation,X-ray radiograph ,histological examination. After 4, 8, 12 weeks ,D-Alk-1B materials, β-TCP and D-Alk-1 B + PRP group had better osteogenesis ability than the experimental control group (P ＜0.05); D-Alk-lB + PRP had the best performance, better than D-Alk-1B and β-TCP (P＜0.05); D-Alk-1B were similar to β-TCP (P＞0.05). D-Alk-1 B materials degradated faster than β-TCP materials, and the porous structure of the materials disappeared after degradation. D-Alk-1B materials intergrated with host's bone was better than β-TCP materials. Conclusion D-Alk-1B material have good biological activity, histocompatibility and biodegradation and simiar presence of bone formation compared withβ-TCP in the aspect of repairing the segmental bone defect, the combination of PRP and D-Alk-1 B strengthened osteogenesis in vivo.
