Effect of propentofylline on NGF and IL-1β release from rat cerebral cortical astrocytes
10.3760/cma.j.issn.0254-1416.2010.05.014
- VernacularTitle:丙戊茶碱对大鼠大脑皮层星形胶质细胞NGF和IL-1β释放的影响
- Author:
Qingcai CHEN
;
Jianping YANG
;
Lina WANG
;
Hao CHENG
;
Yanbing ZHANG
;
Jiying FENG
;
Yan PENG
- Publication Type:Journal Article
- Keywords:
Indoles;
Astrocytes;
Never growth factor;
Interleukins
- From:
Chinese Journal of Anesthesiology
2010;30(5):556-558
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of propentofylline on nerve growth factor (NGF) and IL-1βrelease from rat cerebral cortical astrocytes. Methods Primary cultured rat astrocytes from SD rats (1-3 d,weighing 6-8 g) after 4 passages were randomly divided into 8 groups ( n = 6 wells each): group Ⅰ control (group C); group Ⅱ , Ⅲ, Ⅳ the astrocytes were exposed to propentofylline 10, 100 and 1000 μmol/L respectively (group P1, P2, P3 ); group Ⅴ the astrocytes were exposed to LPS 1 μg/ml and group Ⅵ, Ⅶ, Ⅷ the astrocytes were exposed to propentofylline 10, 100 and 1000 μmol/L in addition to LPS 1 μg/ml (group P1 + LPS, P2 + LPS,P3 + LPS). The astrocytes were then incubated for 3 days in all 8 groups. The concentrations of IL-1β and NGF in the supernatant were detected at 1 and 3 days of incubation using ELISA. Results LPS activated astrocytes resulting in decrease in NGF release and increase in IL-1β release. Propentofylline significantly increased NGF release and decreased IL-1β release from astrocytes incubated alone or with LPS by suppressing activation of astrocytes. Conclusion Propentofylline can enhance NGF release and inhibit IL-1β release from rat cerebral cortical astrocytes.
