Effect of amiloride pretreatment on lipopolysaccharide-induced acute lung injury in rats
10.3760/cma.j.issn.0254-1416.2010.05.028
- VernacularTitle:阿米洛利预先给药对大鼠内毒素性急性肺损伤的影响
- Author:
Hongfei ZHU
;
Ping GUI
;
Shanglong YAO
;
Qingping WU
;
Dan FENG
;
Jin CHENG
- Publication Type:Journal Article
- Keywords:
Amiloride;
Respiratory distress syndrome,adult;
Endotoxemia;
Pretreatment
- From:
Chinese Journal of Anesthesiology
2010;30(5):601-604
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of amiloride pretreatment on the acute lung injury (ALI)induced by lipopolysaccharide (LPS) in rats. Methods Thirty-two pathogen-free male SD rats weighing 200-250 g were randomly divided into 4 groups (n = 8 each); group Ⅰ received iv normal saline (group C); group Ⅱ ALI received iv LPS 6 mg/kg (group ALI); group Ⅲ received iv amiloride 10 mg/kg (group A) and group Ⅳ received amiloride 10 mg/kg iv 30 min before iv LPS ( group AL). The animals were killed by exsanguination at 6 h after iv LPS infusion. The lungs were immediately removed. Microscopic examination of lung tissue was performed. The left lung was lavaged. The total protein (TP), TNF-α and macrophage inflammatory protein-2 (MIP-2)concentrations in broncho-alveolar lavage fluid (BALF) were measured. The W/D weight ratio and the myeloperoxidase (MPO) activity and expression of Na-H exchanger-1 ( NHE1 ), p38MAPK and extracellular signal-regulated kinase (ERK) in lung tissue were determined. Results LPS significantly increased ALI score (0 = slightest, 4 = severest), W/D lung weight ratio, TP, TNF-α and MIP-2 concentrations in BALF and MPO activity and the expression of NHE1, p38MAPK and ERK in the lung as compared with. control group. Amiloride pretreatment significantly attenuated LPS-induced changes except p38MAPK expression. Conclusion Pretreatment with amiloride can attenuate LPS-induced ALI by inhibition of ERK activation.
