Influence of Wnt signaling pathway on mouse NIT-1 β-cell cultured in vitro

VernacularTitle:Wnt信号通路对体外培养小鼠胰岛β细胞的影响
Author: Shuyan GUI ; Muxun ZHANG ; Lili ZHOU ; Yikai YU ; Gang YUAN
Publication Type:Journal Article
Keywords: Wnt-signaling; Islet β-cell; Apoptosis; Proliferation
From: Chinese Journal of Endocrinology and Metabolism 2010;26(8):707-710
CountryChina
Language:Chinese
Abstract: Objective To establish whether Wnt-signaling pathway plays a role in mice β-cell function and/or survival in vitro. Methods Mice NIT-1 beta cells were cultured in media with glucose concentration of 33.3 mmol/L and the cytokines interleukin-1β, interferon-γand tumor necrosis factor-α with or without the addition of purified Wnt3a protein in vitro. Subsequently, β-cell apoptosis by Tunnel and flow cytometry, and β-cell proliferation by BrdU were analyzed. Total RNA was extracted to measure gene expressions by real-time PCR.Results Incubations of NIT-1 cells with high glucose and cytokines resulted in an increase in β-cell apoptosis and decrease in β-cell proliferation (P<0.01). In contrast, treatment with Wnt3a protein protected β-cell from glucose and cytokines-induced apoptosis through up-regulating the expressions of above Pitx2、 TCF7L2. Conclusions Wnt-signaling regulates the proliferation of pancreatic β-cell, and protectes β-cell from glucotoxicity and cytokine toxicity with respect to proliferation and apoptosis.