Primary exploration on identification of pathogenic Trichosporon spp. with rDNA-RFLP analysis

VernacularTitle:rDNA-RFLP多酶切技术用于人类致病相关毛孢子菌种间鉴定初探
Author: Xuelian Lü ; Xiaoli ZHANG ; Miaomiao WANG ; Zehu LIU ; Yongnian SHEN ; Weida LIU
Publication Type:Journal Article
Keywords: Trichosporon; DNA,ribosomal; Polymorphism,restriction fragment length
From: Chinese Journal of Dermatology 2010;43(8):534-537
CountryChina
Language:Chinese
Abstract: Objective To genotype Trichosporon spp. with rDNA-ITSAGSl-RFLP analysis followed by cluster analysis, and attempt to apply this method to rapid species identification of human pathogenic Trichosporon spp.. Methods Fourteen strains of Trichosporon, which belonged to 8 species, were collected. The rDNA-ITS/IGSl regions were amplified by PCR and sequenced. Simultaneously, the amplicons were digested separately with restriction enzymes, including Hae III, Hha I , Hae IH and Hha I , Hinf I , Msp I and Taq I . Results The 8 species of Trichosporon could be classified into 4 subgroups with rDNA-ITS-RFLP, while inter-species identification of all the 14 strains from 8 species of Trichosporon could be realized with rDNA-IGSl-RFLP. Also, those genotypes of T. asahii which had relative long phylogenic distance could even be discriminated with rDNA-IGSl-RFLP. Conclusion The rDNA-ITS/IGSl-RFLP analysis is expected to be used in rapid interspecific identification of genus Trichosporon.