Autophagy in human skin fibroblast model for photoaging

VernacularTitle:培养的人皮肤成纤维细胞光老化模型中自噬水平的研究
Author: Qingsong ZHANG ; Mei JU ; Kun CHEN ; Xinyu LI ; Baozhu CHANG ; Heng GU
Publication Type:Journal Article
Keywords: Autophagy; Skin aging; Fibroblasts
From: Chinese Journal of Dermatology 2010;43(8):572-574
CountryChina
Language:Chinese
Abstract: Objective To observe the changes of autophagy in human skin fibroblast (HSF) model for photoaging. Methods HSF model for photoaging was established through repeated exposure to ultraviolet B (UVB). Those HSFs receiving no irradiation served as the control. The degree of aging was evaluated by p-galactosidase assay, and autophagy level was detected. Results After repeated exposure to UVB, most pho-toaged HSFs were deformed and distorted, and some of them even died. The percentage of P-galactosidase-positive cells was 50.60% ± 5.04% and 14.58% ± 2.69%, respectively in photoaged and control HSFs (P< 0.01). Significant difference was also observed in the proportion of cytophagosome-positive cells between photoaged and control HSFs (14.91% ± 4.59% vs 68.45% ± 8.25%, P < 0.01). Conclusion The HSF model for photoaging shows obviously abnormal appearance and stagnant growth with increased degree of senescence and decreased autophagy compared with normal control HSFs.