Construction of human bone marrow mesenchymal stem cell line genetically modified with human proenkephalin gene

VernacularTitle:人前脑啡肽原基因修饰人骨髓间充质干细胞系的构建
Author: Shaoyan CAI ; Yi SUN ; Guodong ZHAO
Publication Type:Journal Article
Keywords: Enkephalins; Organisms,genetically modified; Stem cells; Bone marrow
From: Chinese Journal of Anesthesiology 2010;30(5):565-568
CountryChina
Language:Chinese
Abstract: Objective To construct h n bone marrow mesenchymal stem cell line genetically modified with human proenkephalin gene. Methods The packaging cell line Phoenix-293T was transfected with the recombinant pBABE-PENK vector to aquire virus. The recombinant virus was then collected and used to infect hMSCs. Stable expression of proenkephalin gene and leucine enkephalin protein and the concentration of leucine enkephalin protein were detected by RT-PCR, immunofluorescence and ELISA respectively. Results The expression of proenkephalin gene and leucine enkephalin protein were significantly up-regulated in the hMSC-PENK cells, and the concentration of leucine enkephalin protein was also increased in the culture medium. Conclusion A human mesenchymal stem cell line that expresses proenkephalin gene and secrets enkephalin was successfully established.