Transcription factor GATA-3 on rats Th2 type cytokine expression regulation after infected by respiratory syncytial virush
10.3760/cma.j.issn.1008-1372.2010.07.016
- VernacularTitle:转录因子GATA-3对呼吸道合胞病毒感染大鼠Th2型细胞因子表达的调控研究
- Author:
Lingli CHEN
;
Xiangrong ZHENG
- Publication Type:Journal Article
- Keywords:
Respiratory syncytial virus infections/ME/IM;
GATA3 transcription factor/ME;
Interleukin-4/ME;
Interleukin-5/ME
- From:
Journal of Chinese Physician
2010;12(7):910-914
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression regulation of GATA-3 on Th2 cytokines secretions in rats lung infected by respiratory syncytial virus (RSV).Methods 20 SD rates were random divided into two groups: Control group (10 rats) and RSV-infected group(10 rats, the model was established by RSV intravasation).The airway responses were observed after seven week.Lung tissue of SD rats were stained with HE, pathologic examinations of lung tissue- stained were made.GATA-3 expressions in lung tissue were detected by western-blots, respectively.Interleukin-4 (IL-4) and interleukin-5 (IL-5) in lung homogenate were detected by ELISA.Results Lung biopsy from RSV-infected group showed typical inflammation under light microscope.Airway resistance in RSV- infected group was higher than control group.Compared with control group( 1.231 ±0.31 ), the expression of GATA-3 significantly increased in RSV-infected group (1.743 ±0.21)( t =15.427, P ＜0.01).The levels of IL-4[(142.3 ± 12.3)ng/L] and IL-5[(167.5 ±14.2) ng/L] increased in RSV-infected group [(69.2±11.6) ng/L, ( 86.6 ± 14.3 ) ng/L] ( P＜0.01 ).There was a positive correlation between protein levels of GATA-3 and IL-4 in lung tissues (r=0.864, P＜0.01 ), and a positive correlation between GATA-3 and IL-5 in lung tissues(r=-0.756, P＜0.01).Conclusion RSV-infection may up regulate the expression of GATA-3 in lung, which contributed to inflammation of Th2 type.
